C7657

Sigma

 

Collagenase from Clostridium histolyticum

release of physiologically active rat pancreatic islets tested, Type XI, 2-5 FALGPA units/mg solid, >1200 CDU/mg solid (CDU = collagen digestion units)

Synonym:Clostridiopeptidase A
CAS Number:9001-12-1
Enzyme Commission (EC) Number:3.4.24.3   ( BRENDA | IUBMB )
EC Number:232-582-9
MDL number:MFCD00130830

Description

Other NotesView more information on collagenase and cell dissociation at www.sigma-aldrich.com/enzymeexplorer.
QualityAlso contains clostripain, nonspecific neutral protease, and tryptic activities.
Unit DefinitionOne collagen digestion unit liberates peptides from collagen equivalent in ninhydrin color to 1.0 μmole of leucine in 5 hr at pH 7.4 at 37 °C in the presence of calcium ions.
One FALGPA hydrolysis unit hydrolyzes 1.0 μmole of furylacryloyl-Leu-Gly-Pro-Ala per min at pH 7.5 at 25 °C in the presence of calcium ions.
One neutral protease unit hydrolyzes casein to produce color equivalent to 1.0 μmole tyrosine per 5 hr at pH 7.5 at 37 °C.
One clostripain unit hydrolyzes 1.0 μmole of BAEE per min at pH 7.6 at 25 °C in the presence of DTT.

Properties

suitabilityrelease of physiologically active rat pancreatic islets tested
storage temp.−20°C

Safety

Personal Protective Equipmentdust mask type N95 (US), Eyeshields, Faceshields, Gloves
Hazard CodesXn
Risk Statements36/37/38-42
Safety Statements22-24-26-36/37
WGK Germany1

Related Products

SubstrateF5135, N-[3-(2-Furyl)acryloyl]-Leu-Gly-Pro-Ala
 C0627, Z-Gly-Pro-Leu-Gly-Pro
InhibitorM3531, (2R)-2-Mercaptomethyl-4-methylpentanoyl-β-(2-naphthyl)-Ala-Ala Amide

References

referenceLacy, P.E., Kostianovsky, M. Diabetes 16, 35, (1967) Abstract
MerckMerck 13,2505